From gene expression analysis to tissue microarrays - A rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies
2006 (English)In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 5, no 6, 1072-1081 p.Article in journal (Refereed) Published
Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy for which better treatment strategies are needed. To identify potential diagnostic and therapeutic targets, a signature consisting of MCL-associated genes was selected based on a comprehensive gene expression analysis of malignant and normal B cells. The corresponding protein epitope signature tags were identified and used to raise monospecific, polyclonal antibodies, which were subsequently analyzed on paraffin-embedded sections of malignant and normal tissue. In this study, we demonstrate that the initial selection strategy of MCL-associated genes successfully allows identification of protein antigens either uniquely expressed or overexpressed in MCL compared with normal lymphoid tissues. We propose that genome-based, affinity proteomics, using protein epitope signature tag-induced antibodies, is an efficient way to rapidly identify a number of disease-associated protein candidates of both previously known and unknown identities.
Place, publisher, year, edition, pages
2006. Vol. 5, no 6, 1072-1081 p.
mantle cell lymphoma, altered apoptosis pathways, follicular lymphoma, molecular diagnosis, cyclin b1, protein, proteomics, survival, signature, cancer
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-15748DOI: 10.1074/mcp.M60077-MCP200ISI: 000238288100009ScopusID: 2-s2.0-33745610852OAI: oai:DiVA.org:kth-15748DiVA: diva2:333790
QC 201411282010-08-052010-08-052014-11-28Bibliographically approved