Change search
ReferencesLink to record
Permanent link

Direct link
Structural basis for detoxification and oxidative stress protection in membranes
Show others and affiliations
2006 (English)In: Journal of Molecular Biology, ISSN 0022-2836, E-ISSN 1089-8638, Vol. 360, no 5, 934-945 p.Article in journal (Refereed) Published
Abstract [en]

Synthesis of mediators of fever, pain and inflammation as well as protection against reactive molecules and oxidative stress is a hallmark of the MAPEG superfamily (membrane associated proteins in eicosanoid and glutathione metabolism). The structure of a MAPEG member, rat mictosomal glutathione transferase 1, at 3.2 angstrom resolution, solved here in complex with glutathione by electron crystallography, defines the active site location and a cytosolic domain involved in enzyme activation. The glutathione binding site is found to be different from that of the canonical soluble glutathione transferases. The architecture of the homotrimer supports a catalytic mechanism involving subunit interactions and reveals both cytosolic and membraneous substrate entry sites, providing a rationale for the membrane location of the enzyme.

Place, publisher, year, edition, pages
2006. Vol. 360, no 5, 934-945 p.
Keyword [en]
membrane protein, oxidative stress, enzymology, protein structure, electron crystallography, microsomal glutathione transferase, leukotriene c-4 synthase, 6 angstrom resolution, s-transferase, electron crystallography, purification, activation, substrate, superfamily, microscopy
URN: urn:nbn:se:kth:diva-15910DOI: 10.1016/j.jmb.2006.05.056ISI: 000239670200002OAI: diva2:333952
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2010-10-04Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Hebert, Hans
By organisation
Structural Biotechnology
In the same journal
Journal of Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 19 hits
ReferencesLink to record
Permanent link

Direct link