Achieving directed immunostimulating complex incorporation
2006 (English)In: Expert Review of Vaccines, ISSN 1476-0584, Vol. 5, no 3, 395-403 p.Article, review/survey (Refereed) Published
In recent years, several studies have been reported with the common aim of generating general expression systems for straightforward production and subsequent coupling of expressed antigens to an adjuvant system. Here, we describe a series of such efforts with a common theme of using gene fusion technology for association of recombinant antigens to immunostimulating complexes (iscoms). In the early stages of vaccine development, uniform antigen preparations are crucial to allow the comparison of immune responses to different antigens, or even subdomains thereof, and we believe that the described systems constitute an important development in this context.
Place, publisher, year, edition, pages
2006. Vol. 5, no 3, 395-403 p.
adjuvant, affinity purification, fusion protein, iscom, subunit vaccine, falciparum antigen pf155/resa, solubilizing fusion partner, direct iscom incorporation, aqueous 2-phase systems, escherichia-coli, recombinant immunogens, bacterial expression, toxoplasma-gondii, protein-a, peptide
IdentifiersURN: urn:nbn:se:kth:diva-16023DOI: 10.1586/147605220.127.116.115ISI: 000240921700017ScopusID: 2-s2.0-33746786432OAI: oai:DiVA.org:kth-16023DiVA: diva2:334065
QC 201005252010-08-052010-08-05Bibliographically approved