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Automated microdroplet platform for sample manipulation and polymerase chain reaction
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Analytical Chemistry.
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2006 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 78, no 22, 7722-7728 p.Article in journal (Refereed) Published
Abstract [en]

We present a fully automated system performing continuous sampling, reagent mixing, and polymerase chain reaction (PCR) in microdroplets transported in immiscible oil. Sample preparation and analysis are totally automated, using an original injection method from a modified 96-well plate layered with three superimposed liquid layers and in-capillary laser-induced fluorescence endpoint detection. The process is continuous, allowing sample droplets to be carried uninterruptedly into the reaction zone while new drops are aspirated from the sample plate. Reproducible amplification, negligible cross-contamination, and detection of low sample concentrations were demonstrated on numerous consecutive sample drops. The system, which opens the route to strong reagents and labor savings in high-throughput applications, was validated on the clinically relevant quantification of progesterone receptor gene expression in human breast cancer cell lines.

Place, publisher, year, edition, pages
2006. Vol. 78, no 22, 7722-7728 p.
Keyword [en]
dna amplification, multiplex pcr, device, system, chip, power
Identifiers
URN: urn:nbn:se:kth:diva-16127DOI: 10.1021/ac061205eISI: 000242021400016Scopus ID: 2-s2.0-33751212797OAI: oai:DiVA.org:kth-16127DiVA: diva2:334169
Note
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2017-12-12Bibliographically approved

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