Process technology for production and recovery of heterologous proteins with Pichia pastoris
2006 (English)In: Biotechnology progress (Print), ISSN 8756-7938, E-ISSN 1520-6033, Vol. 22, no 6, 1465-1473 p.Article, review/survey (Refereed) Published
Developments in process techniques for production and recovery of heterologous proteins with Pichia pastoris are presented. Limitations for the standard techniques are described, and alternative techniques that solve the limitations problems are reviewed together with the methods that resulted in higher productivity of the P. pastoris processes. The main limitations are proteolysis of the secreted products and cell death in the high cell density bioreactor cultures. As a consequence, both low productivity and lower quality of the feedstock for downstream processing are achieved in processes hampered with these problems. Methods for exploring proteolysis and cell death are also presented. Solving the problems makes the conditions for downstream processing superior for the P. pastoris expression systems compared to other systems, which either need complex media or rely on intracellular production. These improved conditions allow for interfacing of cultivation with downstream processing in an integrated fashion.
Place, publisher, year, edition, pages
2006. Vol. 22, no 6, 1465-1473 p.
expanded-bed adsorption, fed-batch fermentation, high-level production, human serum-albumin, recombinant protein, methylotrophic yeast, saccharomyces-cerevisiae, methanol concentration, escherichia-coli, feeding strategy
IdentifiersURN: urn:nbn:se:kth:diva-16165DOI: 10.1021/bp060171tISI: 000242427200001ScopusID: 2-s2.0-33845497166OAI: oai:DiVA.org:kth-16165DiVA: diva2:334207
QC 201005252010-08-052010-08-05Bibliographically approved