Change search
ReferencesLink to record
Permanent link

Direct link
Structurally distinct membrane nanotubes between human macrophages support long-distance vesicular traffic or surfing of bacteria
Show others and affiliations
2006 (English)In: Journal of Immunology, ISSN 0022-1767, E-ISSN 1550-6606, Vol. 177, no 12, 8476-8483 p.Article in journal (Refereed) Published
Abstract [en]

We report that two classes of membrane nanotubes between human monocyte-derived macrophages can be distinguished by their cytoskeletal structure and their functional properties. Thin membrane nanotubes contained only F-actin, whereas thicker nanotubes, i.e., those > similar to 0.7 mu m in diameter, contained both F-actin and microtubules. Bacteria could be trapped and surf along thin, but not thick, membrane nanotubes toward connected macrophage cell bodies. Once at the cell body, bacteria could then be phagocytosed. The movement of bacteria is aided by a constitutive flow of the nanotube surface because streptavidin-coated beads were similarly able to traffic along nanotubes between surface-biotinylated macrophages. Mitochondria. and intracellular vesicles, including late endosomes and lysosomes, could be detected within thick, but not thin, membrane nanotubes. Analysis from kymographs demonstrated that vesicles moved in a stepwise, bidirectional manner at similar to 1 mu m/s, consistent with their traffic being mediated by the microtubules found only in thick nanotubes. Vesicular traffic in thick nanotubes and surfing of beads along thin nanotubes were both stopped upon the addition of azide, demonstrating that both processes require ATP. However, microtubule destabilizing agents colchicine or nocodazole abrogated vesicular transport but not the flow of the nanotube surface, confirming that distinct cytoskeletal structures of nanotubes give rise to different functional properties. Thus, membrane nanotubes between macrophages are more complex than unvarying ubiquitous membrane tethers and facilitate several means for distal interactions between immune cells.

Place, publisher, year, edition, pages
2006. Vol. 177, no 12, 8476-8483 p.
Keyword [en]
natural-killer-cells, dendritic cells, t-cells, intercellular transfer, cytoplasmic dynein, immune cells, in-vivo, retraction, transport, entry
URN: urn:nbn:se:kth:diva-16255ISI: 000243416800024OAI: diva2:334297
QC 20100525Available from: 2010-08-05 Created: 2010-08-05Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Önfelt, Björn
In the same journal
Journal of Immunology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 45 hits
ReferencesLink to record
Permanent link

Direct link