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Association of renal Na,K-ATPase alpha-subunit with the beta- and gamma-subunits based on cryoelectron microscopy
KTH, School of Technology and Health (STH), Structural Biotechnology.ORCID iD: 0000-0002-3220-9402
2006 (English)In: Journal of Membrane Biology, ISSN 0022-2631, E-ISSN 1432-1424, Vol. 214, no 3, 139-146 p.Article in journal (Refereed) Published
Abstract [en]

Na,K-ATPase transports Na+ and K+ across cell membranes and consists of alpha- and beta-subunits. Na,K-ATPase also associates with small FXYD proteins that regulate the activity of the pump. We have used cryoelectron microscopy of two-dimensional crystals including data to 8 A resolution to determine the three-dimensional (3-D) structure of renal Na,K-ATPase containing FXYD2, the gamma-subunit. A homology model for the a- subunit was calculated from a Ca2+-ATPase structure and used to locate the additional beta- and gamma-subunits present in the 3-D map of Na,K-ATPase. Based on the 3-D map, the beta- subunit is located close to transmembrane helices M8 and M10 and the gamma-subunit is adjacent to helices M2 and M9 of the alpha-subunit.

Place, publisher, year, edition, pages
2006. Vol. 214, no 3, 139-146 p.
Keyword [en]
Na, K-ATPase, cryoelectron microscopy, gamma-subunit, beta-subunit, FXYD2, calcium-pump, crystal-structure, 3-dimensional structure, sarcoplasmic-reticulum, cross-links, atpase, resolution, binding, domain, ca2+-atpase
URN: urn:nbn:se:kth:diva-16280DOI: 10.1007/s00232-006-0056-8ISI: 000247991600004ScopusID: 2-s2.0-34447328453OAI: diva2:334322
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2010-10-04Bibliographically approved

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Hebert, Hans
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Structural Biotechnology
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