Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy
2007 (English)In: Optics Express, ISSN 1094-4087, Vol. 15, no 9, 5366-5375 p.Article in journal (Refereed) Published
This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines the advantages of FCS with TIRF microscopy in a way that allows for simultaneous ultra-sensitive spectroscopic measurements using a single-point detector and convenient localization of single molecules on a surface by means of parallel imaging.
Place, publisher, year, edition, pages
2007. Vol. 15, no 9, 5366-5375 p.
high count-rate, molecules, enzyme, fluctuations, diffusion, proteins, glycol)
IdentifiersURN: urn:nbn:se:kth:diva-16617ISI: 000246395000011ScopusID: 2-s2.0-34247886116OAI: oai:DiVA.org:kth-16617DiVA: diva2:334659
QC 201005252010-08-052010-08-05Bibliographically approved