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Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies
KTH, School of Biotechnology (BIO), Bioprocess Technology.
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2007 (English)In: Journal of Biotechnology, ISSN 0168-1656, E-ISSN 1873-4863, Vol. 130, no 3, 291-299 p.Article in journal (Refereed) Published
Abstract [en]

Different cultivation strategies have been compared for the production of Rhizopus oryzae lipase (ROL) from Pichia pastoris. Several drawbacks have been found using a methanol non-limited fed-batch. On the one hand, oxygen limitation appeared at early cell dry weights and, on the other hand, high cell death was observed. A temperature limited fed-batch has been proposed to solve both problems. However, in our case study a methanol non-limited fed-batch results in better productivities. Finally, a lower salt medium were used to overcome cell death problems and a temperature limited fed-batch was applied thereafter to solve oxygen transfer limitations. This combined strategy has resulted in lower productivities when compared to a methanol non-limited fed-batch. However the culture could be longer prolonged and a 1.3-fold purer final product was obtained mainly due to cell death reduction.

Place, publisher, year, edition, pages
2007. Vol. 130, no 3, 291-299 p.
Keyword [en]
Pichia pastoris, operational fed-batch strategies, Rhizopus orysae lipase, cell death, heterologous protein-production, high-level production, recombinant protein, proteolytic degradation, fermentation, expression, methanol, growth, cultivation, viability
Identifiers
URN: urn:nbn:se:kth:diva-16803DOI: 10.1016/j.jbiotec.2007.04.009ISI: 000248109600011Scopus ID: 2-s2.0-34250333764OAI: oai:DiVA.org:kth-16803DiVA: diva2:334846
Note
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2017-12-12Bibliographically approved

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