Photoderivatized polymer thin films at quartz crystal microbalance surfaces: Sensors for carbohydrate-protein interactions
2007 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 79, no 18, 6897-6902 p.Article in journal (Refereed) Published
Photoderivatized polymer-coated gold surfaces have been developed following a perfluorophenylazide-based double ligation strategy. Gold-plated quartz crystal microbalance (QCM) crystals were initially covalently functionalized with a monolayer of poly(ethylene glycol) (PEG), using photo-or thermolytic nitrene formation and insertion. The polymer surfaces were subsequently used as substrates for photoinsertion of carbohydrate-derivatized photoprobes, yielding different recognition motifs for selective protein binding. The resulting robust and biocompatible sensor surfaces were applied to a flow-through QCM instrument for monitoring lectin-carbohydrate interactions in real time. The results clearly show the predicted lectin selectivity, demonstrating the applicability of the approach.
Place, publisher, year, edition, pages
2007. Vol. 79, no 18, 6897-6902 p.
functionalized perfluorophenyl azides, ligand-binding characteristics, lectin concanavalin-a, monomer/dimer equilibrium, glycolipid monolayers, plasmon resonance, mistletoe lectin, pisum-sativum, affinity, mannan
IdentifiersURN: urn:nbn:se:kth:diva-16958DOI: 10.1021/ac070740rISI: 000249527700001ScopusID: 2-s2.0-34548783269OAI: oai:DiVA.org:kth-16958DiVA: diva2:335001
QC 201005252010-08-052010-08-052012-03-22Bibliographically approved