Intracellular dynamics of calcyon, a neuron-specific vesicular protein
2007 (English)In: NeuroReport, ISSN 0959-4965, E-ISSN 1473-558X, Vol. 18, no 15, 1547-1551 p.Article in journal (Refereed) Published
Calcyon is a brain-specific protein, implicated in clathrin-mediated endocytosis. In this descriptive study we show that calcyon is exclusively expressed in neurons, and localized in moving vesicles. The movement of calcyon-containing vesicles was dependent on temperature and on intact microtubules, in addition these vesicles were colocalized with a marker for endocytosed plasma membrane proteins, suggesting that calcyon vesicles follow the endocytic recycling pathway. We also show using evanescent wave microscopy that there is a pool of ready releasable calcyon vesiclesaccumulated beneath the plasma membrane. We conclude that the mobility and storage properties of calcyon-containing vesicles imply that they play a role in brain plasticity.
Place, publisher, year, edition, pages
2007. Vol. 18, no 15, 1547-1551 p.
calcyon, endocytic recycling pathway, total internal reflection fluorescence microscopy, vesicles, receptors, endocytosis, activation, expression, spines, brain, ltp
IdentifiersURN: urn:nbn:se:kth:diva-16996ISI: 000249853200009ScopusID: 2-s2.0-34748818773OAI: oai:DiVA.org:kth-16996DiVA: diva2:335039
QC 201005252010-08-052010-08-05Bibliographically approved