The physical action of cellulases revealed by a quartz crystal microbalance study using ultrathin cellulose films and pure
2008 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 9, no 1, 249-254 p.Article in journal (Refereed) Published
The effects of fungal cellulases on model cellulose films were studied using a high-resolution quartz crystal microbalance (QCM) sensitive to minute changes of the nanometer thick model cellulose films. It was found that endoglucanases not only produce new end groups but also cause a swelling of the cellulose film. The cellobiohydrolases degraded the films quickly, which was detected as a rapid decrease in the remaining amount of cellulose on the QCM crystal. However, changing viscoelastic properties of the films also indicated a softening of the film during the degradation. A defined mixture of selected cellulases caused a significantly higher rate of degradation than only cellobiohydrolases. Cellulase synergism is discussed with the endoglucanase swelling effects and film softening added.
Place, publisher, year, edition, pages
2008. Vol. 9, no 1, 249-254 p.
trichoderma-reesei, phanerochaete-chrysosporium, binding domains, model, films, degradation, mechanism, synergism
IdentifiersURN: urn:nbn:se:kth:diva-17271DOI: 10.1021/bm700980bISI: 000252415600034ScopusID: 2-s2.0-38849118325OAI: oai:DiVA.org:kth-17271DiVA: diva2:335314
QC 201005252010-08-052010-08-05Bibliographically approved