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Diode-pumped solid state laser light sources for confocal laser scanning fluorescence microscopy
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Analytical Chemistry (closed 20110630).
KTH, School of Engineering Sciences (SCI), Applied Physics, Laser Physics.ORCID iD: 0000-0001-7688-1367
2008 (English)In: Journal of laser applications, ISSN 1042-346X, Vol. 20, no 3, 160-164 p.Article in journal (Refereed) Published
Abstract [en]

Diode-pumped solid-state lasers (DPSSLs) have been integrated as light sources in confocal laser scanning microscopy (CLSM). The standard argon ion laser at 488 rim is compared with a DPSSL operating at 473 run in terms of noise and CLSM image characteristics. The equally high fluorescence image quality together with the many advantageous characteristics inherent to solid-state lasers suggest that excitation using a DPSSL is favored. The application of a dual-line DPSSL emitting at 491 and 532 nm for high resolution CLSM fluorescence imaging is shown for the first time.

Place, publisher, year, edition, pages
2008. Vol. 20, no 3, 160-164 p.
URN: urn:nbn:se:kth:diva-17838ISI: 000259388200005ScopusID: 2-s2.0-57049152933OAI: diva2:335883
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2015-03-30Bibliographically approved

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Stjernström, MårtenLaurell, Fredrik
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