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MAP20, a Microtubule-Associated Protein in the Secondary Cell Walls of Hybrid Aspen, Is a Target of the Cellulose Synthesis Inhibitor 2,6-Dichlorobenzonitrile
KTH, School of Biotechnology (BIO), Centres, Swedish Center for Biomimetic Fiber Engineering, BioMime.
KTH, School of Biotechnology (BIO), Centres, Swedish Center for Biomimetic Fiber Engineering, BioMime.ORCID iD: 0000-0002-8576-4370
KTH, School of Biotechnology (BIO), Centres, Swedish Center for Biomimetic Fiber Engineering, BioMime.
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2008 (English)In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 148, no 3, 1283-1294 p.Article in journal (Refereed) Published
Abstract [en]

We have identified a gene, denoted PttMAP20, which is strongly up-regulated during secondary cell wall synthesis and tightly coregulated with the secondary wall-associated CESA genes in hybrid aspen (Populus tremula x tremuloides). Immunolocalization studies with affinity-purified antibodies specific for PttMAP20 revealed that the protein is found in all cell types in developing xylem and that it is most abundant in cells forming secondary cell walls. This PttMAP20 protein sequence contains a highly conserved TPX2 domain first identified in a microtubule-associated protein (MAP) in Xenopus laevis. Overexpression of PttMAP20 in Arabidopsis (Arabidopsis thaliana) leads to helical twisting of epidermal cells, frequently associated with MAPs. In addition, a PttMAP20-yellow fluorescent protein fusion protein expressed in tobacco (Nicotiana tabacum) leaves localizes to microtubules in leaf epidermal pavement cells. Recombinant PttMAP20 expressed in Escherichia coli also binds specifically to in vitro-assembled, taxol-stabilized bovine microtubules. Finally, the herbicide 2,6-dichlorobenzonitrile, which inhibits cellulose synthesis in plants, was found to bind specifically to PttMAP20. Together with the known function of cortical microtubules in orienting cellulose microfibrils, these observations suggest that PttMAP20 has a role in cellulose biosynthesis.

Place, publisher, year, edition, pages
2008. Vol. 148, no 3, 1283-1294 p.
Keyword [en]
carbohydrate-active enzymes, plant functional genomics, kinesin-like, protein, cortical microtubules, plasma-membrane, wood formation, arabidopsis-thaliana, conifer tracheids, angiosperm trees, urea solution
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:kth:diva-17951DOI: 10.1104/pp.108.121913ISI: 000260719500010Scopus ID: 2-s2.0-57749101097OAI: oai:DiVA.org:kth-17951DiVA: diva2:335996
Note

QC 20100525

Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Functional genomics of wood degradation and biosynthesis
Open this publication in new window or tab >>Functional genomics of wood degradation and biosynthesis
2005 (English)Licentiate thesis, comprehensive summary (Other scientific)
Abstract [en]

Forest biotechnology is a fast emerging field of research. The application of biotechnological tools will enhance the quality of the forest products. The resultant value added and environmentally sustainable products are an absolute necessity in the future. The study of wood biosynthesis and degradation will result in enormous knowledge resources, which can be used for exploiting wood properties. This thesis addresses questions representing both wood degradation and biosynthesis.

The wood degrading fungus Phanerochaete chrysosporium is expression profiled with the microarray technology. The objective is to understand the expression pattern of the extracellular carbohydrate active enzymes (CAZymes) secreted by the organism. The data obtained increases our understanding of gene expression upon growth on cellulose.

Wood biosynthesis is studied with the model wood forming tree species, Populus. The plentiful data resources from the expression profiling during wood formation in Populus are used as the platform of this work. One of the wood specific genes, PttMAP20, previously with an unknown function is studied in this thesis. The immunolocalisation of PttMAP20 with specific antibodies is demonstrated. The putative microtubule-targeting domain of the protein is demonstrated microscopically and by using a biochemical binding assay.

Place, publisher, year, edition, pages
Stockholm: KTH, 2005. 42 p.
Keyword
populus, xylogenesis, secondary cell wall, cellulose, hemicellulose, microarrays, transcript profiling
National Category
Other Biological Topics
Identifiers
urn:nbn:se:kth:diva-592 (URN)91-7178-250-8 (ISBN)
Presentation
2006-01-30, Sal FB54, AlbaNova, Roslagstullsbacken, Stockholm, 10:00
Opponent
Supervisors
Note
QC 20101217Available from: 2006-01-16 Created: 2006-01-16 Last updated: 2011-11-23Bibliographically approved

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Aspeborg, HenrikHober, SophiaDivne, ChristinaEzcurra, Inés

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Swedish Center for Biomimetic Fiber Engineering, BioMimeComputational Biology, CBProteomics (closed 20130101)Glycoscience
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