Non-contact protein microarray fabrication using a procedure based on liquid bridge formation
2009 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 393, no 2, 591-598 p.Article in journal (Refereed) Published
Contemporary microarrayers of contact or noncontact format used in protein microarray fabrication still suffer from a number of problems, e. g. generation of satellite spots, inhomogeneous spots, misplaced or even absent spots, and sample carryover. In this paper, a new concept of non-contact sample deposition that reduces such problems is introduced. To show the potential and robustness of this pressure-assisted deposition technique, different sample solutions known to cause severe problems or to be even impossible to print with conventional microarrayers were accurately printed. The samples included 200 mg mL(-1) human serum albumin, highly concentrated sticky cell adhesion proteins, pure high-salt cell-lysis buffer, pure DMSO, and a suspension of 5-mu m polystyrene beads. Additionally, a water-immiscible liquid fluorocarbon, which was shown not to affect the functionality of the capture molecules, was employed as a lid to reduce evaporation during microarray printing. The fluorocarbon liquid lid was shown to circumvent hydrolysis of water-sensitive activated surfaces during long-term deposition procedures.
Place, publisher, year, edition, pages
2009. Vol. 393, no 2, 591-598 p.
IdentifiersURN: urn:nbn:se:kth:diva-18066DOI: 10.1007/s00216-008-2509-7ISI: 000261936700022ScopusID: 2-s2.0-58049213792OAI: oai:DiVA.org:kth-18066DiVA: diva2:336112
QC 201005252010-08-052010-08-052011-02-07Bibliographically approved