On the role of the monolignol gamma-carbon functionality in lignin biopolymerization
2009 (English)In: Phytochemistry, ISSN 0031-9422, E-ISSN 1873-3700, Vol. 70, no 1, 147-155 p.Article in journal (Refereed) Published
In order to investigate the importance of the monomeric gamma-carbon chemistry in lignin biopolymerization and structure, synthetic lignins (dehydrogenation polymers; DHP) were made from monomers with different degrees of oxidation at the gamma-carbon, i.e., carboxylic acid, aldehyde and alcohol. All monomers formed a polymeric material through enzymatic oxidation. The polymers displayed similar sizes by size exclusion chromatography analyses, but also exhibited some physical and chemical differences. The DHP made of coniferaldehyde had poorer solubility properties than the other DHPs, and through contact angle of water measurement on spin-coated surfaces of the polymeric materials, the DHPs made of coniferaldehyde and carboxylic ferulic acid exhibited higher hydrophobicity than the coniferyl alcohol DHP. A structural characterization with C-13 NMR revealed major differences between the coniferyl alcohol-based polymer and the coniferaldehyde/ferulic acid polymers, such as the predominance of aliphatic double bonds and the lack of certain benzylic structures in the latter cases. The biological role of the reduction at the gamma-carbon during monolignol biosynthesis with regard to lignin polymerization is discussed.
Place, publisher, year, edition, pages
2009. Vol. 70, no 1, 147-155 p.
Lignin, Monolignol biosynthesis, Lignin-polysaccharide networks, Plant, cell wall, Biopolymer, spruce wood, carbohydrate complexes, biosynthesis, dehydrogenation, polymerization, peroxidase, coniferyl, plants
IdentifiersURN: urn:nbn:se:kth:diva-18244DOI: 10.1016/j.phytochem.2008.10.014ISI: 000264235800019ScopusID: 2-s2.0-59049107798OAI: oai:DiVA.org:kth-18244DiVA: diva2:336290
QC 20100525. Uppdaterad från manuskript till artikel (20100811). Tidigare titel: On the role of the monolignol gamma-carbon in lignin biopolymerization2010-08-052010-08-052011-02-09Bibliographically approved