Detection and Analysis of Low-Abundance Cell-Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets
2009 (English)In: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 48, no 14, 2518-2521 p.Article in journal (Refereed) Published
Finding the few: Cell-surface proteins are useful disease biomarkers, but current high-throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high-throughput method for detection and analysis of cell-surface biomarkers expressed at very low levels on individual human cells. Droplet optical labels allow concurrent analysis of several samples.
Place, publisher, year, edition, pages
2009. Vol. 48, no 14, 2518-2521 p.
droplets, enzymatic amplification, high-throughput screening, microfluidics, proteins, polymerase-chain-reaction, protein expression, picoliter droplets, single cells, devices, microdroplets, encapsulation, molecules, therapy, gene
IdentifiersURN: urn:nbn:se:kth:diva-18305DOI: 10.1002/anie.200804326ISI: 000264661600014ScopusID: 2-s2.0-70349782198OAI: oai:DiVA.org:kth-18305DiVA: diva2:336351
QC 201005252010-08-052010-08-052011-02-25Bibliographically approved