Change search
ReferencesLink to record
Permanent link

Direct link
Live Cell Linear Dichroism Imaging Reveals Extensive Membrane Ruffling within the Docking Structure of Natural Killer Cell Immune Synapses
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.
Show others and affiliations
2009 (English)In: Biophysical Journal, ISSN 0006-3495, E-ISSN 1542-0086, Vol. 96, no 2, L13-L15 p.Article in journal (Refereed) Published
Abstract [en]

We have applied fluorescence imaging of two-photon linear dichroism to measure the subresolution organization of the cell membrane during formation of the activating (cytolytic) natural killer (NK) cell immune synapse (IS). This approach revealed that the NK cell plasma membrane is convoluted into ruffles at the periphery, but not in the center of a mature cytolytic NK cell IS. Time-lapse imaging showed that the membrane ruffles formed at the initial point of contact between NK cells and target cells and then spread radialy across the intercellular contact as the size of the IS increased, becoming absent from the center of the mature synapse. Understanding the role of such extensive membrane ruff ling in the assembly of cytolytic synapses is an intriguing new goal.

Place, publisher, year, edition, pages
2009. Vol. 96, no 2, L13-L15 p.
Keyword [en]
microtubule-organizing center, fluorescence, polarization, orientation, activation
URN: urn:nbn:se:kth:diva-18450DOI: 10.1016/j.bpj.2008.10.005ISI: 000266377200003ScopusID: 2-s2.0-58849107192OAI: diva2:336497
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2010-12-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Vanherberghen, BrunoÖnfelt, Björn
By organisation
Cell Physics
In the same journal
Biophysical Journal

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 32 hits
ReferencesLink to record
Permanent link

Direct link