Live Cell Linear Dichroism Imaging Reveals Extensive Membrane Ruffling within the Docking Structure of Natural Killer Cell Immune Synapses
2009 (English)In: Biophysical Journal, ISSN 0006-3495, E-ISSN 1542-0086, Vol. 96, no 2, L13-L15 p.Article in journal (Refereed) Published
We have applied fluorescence imaging of two-photon linear dichroism to measure the subresolution organization of the cell membrane during formation of the activating (cytolytic) natural killer (NK) cell immune synapse (IS). This approach revealed that the NK cell plasma membrane is convoluted into ruffles at the periphery, but not in the center of a mature cytolytic NK cell IS. Time-lapse imaging showed that the membrane ruffles formed at the initial point of contact between NK cells and target cells and then spread radialy across the intercellular contact as the size of the IS increased, becoming absent from the center of the mature synapse. Understanding the role of such extensive membrane ruff ling in the assembly of cytolytic synapses is an intriguing new goal.
Place, publisher, year, edition, pages
2009. Vol. 96, no 2, L13-L15 p.
microtubule-organizing center, fluorescence, polarization, orientation, activation
IdentifiersURN: urn:nbn:se:kth:diva-18450DOI: 10.1016/j.bpj.2008.10.005ISI: 000266377200003ScopusID: 2-s2.0-58849107192OAI: oai:DiVA.org:kth-18450DiVA: diva2:336497
QC 201005252010-08-052010-08-052010-12-14Bibliographically approved