High-resolution computed tomography with a compact soft x-ray microscope
2009 (English)In: Optics Express, ISSN 1094-4087, Vol. 17, no 13, 11057-11065 p.Article in journal (Refereed) Published
Computed tomography based on high-resolution soft x-ray microscopy utilizes the natural contrast for biological specimens provided by the water window (lambda = 2.4 - 4.4 nm) and the high resolving power of zone plate objectives. It is capable of revealing the 3D structure of biological specimens at sub-visible-microscopic resolution. To date, the technique has only been available at synchrotron-based microscopes, which limits the researchers access. In the present paper we demonstrate high-resolution soft x-ray tomography with a laboratory zone-plate-based soft x-ray microscope. The specimen, a diatom mounted on a glass capillary, was reconstructed from a tilt series of 53 images covering 180 using a filtered back projection algorithm. The resolution of the tomogram was estimated to a half period of 140 nm using a differential-phase-residual method. Cryo-fixation, increased source brightness and extended-depth-of-focus objectives are important for pushing the resolution of compact systems for biological samples.
Place, publisher, year, edition, pages
2009. Vol. 17, no 13, 11057-11065 p.
IdentifiersURN: urn:nbn:se:kth:diva-18584ISI: 000267761100066ScopusID: 2-s2.0-67649271251OAI: oai:DiVA.org:kth-18584DiVA: diva2:336631
QC 201005252010-08-052010-08-052011-02-21Bibliographically approved