An Electrochemical Sensor Array and Its Application to Real-Time Brain Slice Imaging
2009 (English)In: Electronics and Communications in Japan, ISSN 1942-9533, Vol. 92, no 9, 217-221 p.Article in journal (Refereed) Published
An electrochemical sensing system using a planar microelectrode array has been developed for monitoring biological molecules with relatively high spatial and temporal resolution. This allows noninvasive real-time imaging of biological molecules released from tissue. In this study, we establish a multichannel hydrogen peroxide (H2O2) sensing system to monitor the real-time H2O2 distribution in tissue using a planar sensor array. H2O2 is known to be associated with the pathology of neurological diseases because it is a by-product of degenerative reactions involving reactive oxygen species, which are a major cause of oxidative stress in mammalian cells. The sensor array is based on a 64-channel ITO electrode array composed of 50 x 50 mu m electrodes modified with an enzyme (horseradish peroxidase) and an electron transfer mediator. We place a cultured rat hippocampal slice on the array and measure the current at each sensor using a multipotentiostat. When we introduce bicuculline into the solution as a stimulant in the presence of a catalase inhibitor, we can observe a distinct increase in the H2O2 concentration. This real-time H2O2 distribution monitoring system is a powerful tool with which to explore the neuronal cell death mechanism in biological systems.
Place, publisher, year, edition, pages
2009. Vol. 92, no 9, 217-221 p.
Electrochemical sensor, Hippocampal slice, Hydrogen peroxide, Microelectrode array
Engineering and Technology
IdentifiersURN: urn:nbn:se:kth:diva-18735DOI: 10.1002/ecj.10140ISI: 000269596300001ScopusID: 2-s2.0-70249088006OAI: oai:DiVA.org:kth-18735DiVA: diva2:336782
QC 20100525 QC 201202072010-08-052010-08-052012-02-07Bibliographically approved