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The RY/Sph element mediates transcriptional repression of maturation genes from late maturation to early seedling growth
KTH, School of Biotechnology (BIO), Centres, Swedish Center for Biomimetic Fiber Engineering, BioMime.
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2009 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 184, no 3, 552-565 p.Article in journal (Refereed) Published
Abstract [en]

P>In orthodox seeds, the transcriptional activator ABI3 regulates two major stages in embryo maturation: a mid-maturation (MAT) stage leading to accumulation of storage compounds, and a late maturation (LEA) stage leading to quiescence and desiccation tolerance. Our aim was to elucidate mechanisms for transcriptional shutdown of MAT genes during late maturation, to better understand phase transition between MAT and LEA stages. Using transgenic and transient approaches in Nicotiana, we examined activities of two ABI3-dependent reporter genes driven by multimeric RY and abscisic acid response elements (ABREs) from a Brassica napus napin gene, termed RY and ABRE, where the RY reporter requires ABI3 DNA binding. Expression of RY peaks during mid-maturation and drops during late maturation, mimicking the MAT gene program, and in Arabidopsis thaliana RY elements are over-represented in MAT, but not in LEA, genes. The ABI3 transactivation of RY is inhibited by staurosporine, by a PP2C phosphatase, and by a repressor of maturation genes, VAL1/HSI2. The RY element mediates repression of MAT genes, and we propose that transcriptional shutdown of the MAT program during late maturation involves inhibition of ABI3 DNA binding by dephosphorylation. Later, during seedling growth, VAL1/HSI2 family repressors silence MAT genes by binding RY elements. New Phytologist (2009)doi: 10.1111/j.1469-8137.2009.02977.x.

Place, publisher, year, edition, pages
2009. Vol. 184, no 3, 552-565 p.
Keyword [en]
ABI3, embryo maturation, gene expression, repression, RY element, storage proteins, conserved b3 domain, abscisic-acid, dna-binding, arabidopsis-thaliana, g-box, regulatory networks, late embryogenesis, active repression, expression, protein
Identifiers
URN: urn:nbn:se:kth:diva-18875DOI: 10.1111/j.1469-8137.2009.02977.xISI: 000270902200006Scopus ID: 2-s2.0-70350132711OAI: oai:DiVA.org:kth-18875DiVA: diva2:336922
Note
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2017-12-12Bibliographically approved

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