Modelling and sensitivity analysis of the reactions involving receptor, G-protein and effector in vertebrate olfactory receptor neurons
2009 (English)In: Journal of Computational Neuroscience, ISSN 0929-5313, E-ISSN 1573-6873, Vol. 27, no 3, 471-491 p.Article in journal (Refereed) Published
A biochemical model of the receptor, G-protein and effector (RGE) interactions during transduction in the cilia of vertebrate olfactory receptor neurons (ORNs) was developed and calibrated to experimental recordings of cAMP levels and the receptor current (RC). The model describes the steps from odorant binding to activation of the effector enzyme which catalyzes the conversion of ATP to cAMP, and shows how odorant stimulation is amplified and delayed by the RGE transduction cascade. A time-dependent sensitivity analysis was performed on the model. The model output-the cAMP production rate-is particularly sensitive to a few, dominant parameters. During odorant stimulation it depends mainly on the initial density of G-proteins and the catalytic constant for cAMP production.
Place, publisher, year, edition, pages
2009. Vol. 27, no 3, 471-491 p.
Olfaction, Receptor neuron, Biochemical model, Sensitivity analysis, Receptor potential, Signal transduction, nucleotide-gated conductance, cyclic-amp, computational model, adenylyl-cyclase, transduction, cilia, cells, adaptation, odorants, calcium
IdentifiersURN: urn:nbn:se:kth:diva-18891DOI: 10.1007/s10827-009-0162-6ISI: 000271105700010ScopusID: 2-s2.0-70350569929OAI: oai:DiVA.org:kth-18891DiVA: diva2:336938
QC 201005252010-08-052010-08-052011-02-07Bibliographically approved