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Lorentzian spatial intensity distribution in one-photon fluorescence correlation spectroscopy
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0002-5584-9170
KTH, School of Information and Communication Technology (ICT), Microelectronics and Applied Physics, MAP.ORCID iD: 0000-0002-2082-9583
2009 (English)In: Applied Optics, ISSN 0003-6935, E-ISSN 1539-4522, Vol. 48, no 31, 6050-6058 p.Article in journal (Refereed) Published
Abstract [en]

The theory of autocorrelation-function evaluation in fluorescence correlation spectroscopy is applied to a Lorentzian intensity distribution. An analytical solution to the autocorrelation function for diffusion is deduced for this spatial distribution. Experimental investigation of the distribution is performed using an enlarged detector aperture in a standard confocal setup. The data from the experiment are fitted to the derived autocorrelation function, and a reasonable estimate of the spatial distribution is provided. Estimates are also compared to values computed by molecular detection efficiency simulation. The use of Lorentzian intensity distributions complements conditions where a Gaussian intensity distribution applies, expanding the applicability range of analytical correlation functions.

Place, publisher, year, edition, pages
2009. Vol. 48, no 31, 6050-6058 p.
Keyword [en]
internal-reflection fluorescence, mode wave-guides, high count-rate, fluctuation spectroscopy, detection volumes, diffusion, dynamics, probe, microscopy, artifacts
URN: urn:nbn:se:kth:diva-18927ISI: 000271374000047ScopusID: 2-s2.0-70449623657OAI: diva2:336974
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2011-02-17Bibliographically approved

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