Inverse-Fluorescence Correlation Spectroscopy
2009 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 81, no 22, 9209-9215 p.Article in journal (Refereed) Published
An alternative version of fluorescence correlation spectroscopy is presented, where the signal from a medium surrounding the particles of interest is analyzed, as opposed to a signal from the particles themselves. Ibis allows for analysis of unlabeled particles and potentially of biomolecules. Here, the concept together with principal experiments on polystyrene beads of 100, 200, 400, and 800 nm diameter in an aqueous solution of alexa 488-fluorophores are presented. The use of photo detectors allowing higher photon fluxes, or of reduced detection volumes, should enable analysis of significantly smaller particles or even biomolecules.
Place, publisher, year, edition, pages
2009. Vol. 81, no 22, 9209-9215 p.
detection technology, diffusion
IdentifiersURN: urn:nbn:se:kth:diva-18945DOI: 10.1021/ac9010205ISI: 000271662400002ScopusID: 2-s2.0-70649097389OAI: oai:DiVA.org:kth-18945DiVA: diva2:336992
QC 201005252010-08-052010-08-052014-06-09Bibliographically approved