Photo-Click Immobilization of Carbohydrates on Polymeric Surfaces - A Quick Method to Functionalize Surfaces for Biomolecular Recognition Studies
2009 (English)In: Bioconjugate chemistry, ISSN 1043-1802, E-ISSN 1520-4812, Vol. 20, no 12, 2364-2370 p.Article in journal (Refereed) Published
Methods to rapidly functionalize specific polymeric surfaces with alkynes, which can subsequently be linked to azide-containing carbohydrates, are presented. The methods comprise two main concepts: azide photoligation and Cu-catalyzed azide-alkyne cycloaddition. 2-Azidoethyl-functionalized CL-D-mannopyranoside was synthesized and covalently attached to alkyne-functionalized polymeric surfaces using the techniques. The protein recognition properties of the carbollydrate-presenting surfaces were evaluated using quartz crystal microbalance biosensor instrumentation.
Place, publisher, year, edition, pages
2009. Vol. 20, no 12, 2364-2370 p.
IdentifiersURN: urn:nbn:se:kth:diva-19043DOI: 10.1021/bc9003519ISI: 000272690100021PubMedID: 19888719ScopusID: 2-s2.0-72449192287OAI: oai:DiVA.org:kth-19043DiVA: diva2:337090
QC 201005252010-08-052010-08-052016-07-21Bibliographically approved