Osteogenic Differentiation by Rat Bone Marrow Stromal Cells on Customized Biodegradable Polymer Scaffolds
2010 (English)In: Journal of bioactive and compatible polymers (Print), ISSN 0883-9115, E-ISSN 1530-8030, Vol. 25, no 2, 207-223 p.Article in journal (Refereed) Published
In this report, poly(L-lactide-co-epsilon-caprolactone), poly(LLA-co-CL) and poly(L-lactide-co-1,5-dioxepan-2-one), poly(LLA-co-DXO) were evaluated and compared for potential use in bone tissue engineering constructs together with bone marrow stromal cells (BMSC). The copolymers were tailored to reduce the level of harmful tin residuals in the scaffolding. BMSC isolated from Sprague-Dawley rats were seeded onto the scaffolds and cultured in vitro for up to 21 days. Cell spreading and proliferation was analyzed after 72 h by scanning electron microscopy and thiazolyl blue tetrazolium bromide (MTT) conversion assay. Osteogenic differentiation of BMSC was evaluated by real-time PCR after 14 and 21 days of culture. Hydrophilicity was significantly different between poly(LLA-co-CL) and poly(LLA-co-DXO) with the latter being more hydrophilic. After 72 h, both scaffolds supported increased cell proliferation and the mRNA expression of osteocalcin and osteopontin was significantly increased after 21 days. Further investigation of these constructs, with lower levels of tin residuals, are being pursued.
Place, publisher, year, edition, pages
2010. Vol. 25, no 2, 207-223 p.
biopolymers, bone marrow stromal cells, degradable scaffold, tissue, engineering, cell proliferation, poly(L-lactide-co-epsilon-caprolactone), poly(L-lactide-co-1, 5-dioxepan-2-one), low tin residuals, mesenchymal stem-cells, of-the-art, in-vitro, aliphatic polyesters, porous scaffolds, acid) foams, osteoblast, poly(l-lactide), degradation, copolymers
Industrial Biotechnology Materials Engineering
IdentifiersURN: urn:nbn:se:kth:diva-19210DOI: 10.1177/0883911509358812ISI: 000274532200006ScopusID: 2-s2.0-77749245896OAI: oai:DiVA.org:kth-19210DiVA: diva2:337257
QC 201101312010-08-052010-08-052011-11-11Bibliographically approved