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Characterization of exopolysaccharides in marine colloids by capillary electrophoresis with indirect UV detection
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Analytical Chemistry.
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Analytical Chemistry.ORCID iD: 0000-0002-3444-9987
2010 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 662, no 2, 193-199 p.Article in journal (Refereed) Published
Abstract [en]

A method was established using capillary electrophoresis with indirect UV detection for analysis of monosaccharides liberated from exopolysaccharides by acidic hydrolysis. Tangential flow filtration was used to isolate high molecular weight polysaccharides from seawater. The capillary electrophoresis method included the use of a background electrolyte consisting of 2,6-dimethoxyphenol and cetyltrimethylammonium bromide. Several neutral sugars commonly existing in marine polysaccharides were separated under optimized conditions. The relative standard deviations were between 1.3% and 2.3% for relative migration time and 1.3-2.5% for peak height. Detection limits (at S/N 3) were in the range of 27.2-47.8 mu M. The proposed approach was applied to the analysis of hydrolyzed colloidal polysaccharides in seawater collected from the Baltic Sea. Nanomolar levels of liberated monosaccharides in seawater samples can be detected by preconcentration up to 30,000 times.

Place, publisher, year, edition, pages
2010. Vol. 662, no 2, 193-199 p.
Keyword [en]
Exopolysaccharide, Capillary electrophoresis, Extracellular polymeric substances, Marine colloid
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-19295DOI: 10.1016/j.aca.2010.01.008ISI: 000275340600010OAI: oai:DiVA.org:kth-19295DiVA: diva2:337342
Funder
Swedish Research CouncilKnut and Alice Wallenberg Foundation
Note
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2017-12-12Bibliographically approved

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Emmer, Åsa

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