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Analysis of the p53 tumor suppressor gene by pyrosequencing
KTH, Superseded Departments, Biotechnology.
KTH, Superseded Departments, Biotechnology.ORCID iD: 0000-0003-4313-1601
KTH, Superseded Departments, Biochemistry and Biotechnology.
KTH, Superseded Departments, Biotechnology.ORCID iD: 0000-0001-8993-048X
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2000 (English)In: BioTechniques, ISSN 0736-6205, E-ISSN 1940-9818, Vol. 28, no 1, 140-+ p.Article in journal (Refereed) Published
Abstract [en]

Tumor suppressor genes are implicated in cell cycle progression. Inactivation of these genes predominantly occurs through mutations and/or allelic loss that involves both alleles. With inactivation by multiple mutations in a single gene, cloning of the amplified gene is necessary to determine whether the mutations reside on one ol both alleles. Using pyrosequencing, a recently developed approach based on sequencing-by-synthesis, we studied genetic variability in the p53 tumor suppressor gene and could quantify the ratio between the mutated and wild-type amplified fragments. Further-more, this sequencing technique also allows allelic determination of adjacent mutations with no cloning of amplified fragments.

Place, publisher, year, edition, pages
2000. Vol. 28, no 1, 140-+ p.
Keyword [en]
cancer, mutations
Identifiers
URN: urn:nbn:se:kth:diva-19480ISI: 000084717700025OAI: oai:DiVA.org:kth-19480DiVA: diva2:338172
Note
QC 20100525Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2017-12-12Bibliographically approved

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Uhlén, Mathias

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