Analysis of the p53 tumor suppressor gene by pyrosequencing
2000 (English)In: BioTechniques, ISSN 0736-6205, Vol. 28, no 1, 140-+ p.Article in journal (Refereed) Published
Tumor suppressor genes are implicated in cell cycle progression. Inactivation of these genes predominantly occurs through mutations and/or allelic loss that involves both alleles. With inactivation by multiple mutations in a single gene, cloning of the amplified gene is necessary to determine whether the mutations reside on one ol both alleles. Using pyrosequencing, a recently developed approach based on sequencing-by-synthesis, we studied genetic variability in the p53 tumor suppressor gene and could quantify the ratio between the mutated and wild-type amplified fragments. Further-more, this sequencing technique also allows allelic determination of adjacent mutations with no cloning of amplified fragments.
Place, publisher, year, edition, pages
2000. Vol. 28, no 1, 140-+ p.
IdentifiersURN: urn:nbn:se:kth:diva-19480ISI: 000084717700025OAI: oai:DiVA.org:kth-19480DiVA: diva2:338172
QC 201005252010-08-102010-08-10Bibliographically approved