The relative importance of intracellular proteolysis and transport on the yield of the periplasmic enzyme penicillin amidase in Escherichia coli
2000 (English)In: Enzyme and microbial technology, ISSN 0141-0229, E-ISSN 1879-0909, Vol. 26, no 04-feb, 165-170 p.Article in journal (Refereed) Published
Intracellular proteolysis is an important mechanism for regulating the level of the periplasmic enzyme penicillin amidase in Escherichia coli. Evidence is presented that the active enzyme is localized in the periplasmic space and maturation of pro-enzyme occurs during transport through the cytoplasmic membrane or rapidly after its entrance in the periplasm. The rate constants of the transport through cytoplasmic membrane and of the intracellular proteolysis were estimated to be 0.01 h and 0.5 h, respectively. This indicates that more than 90% of the synthesized pre-pro-enzyme is lost by intracellular proteolysis occurring in the cytoplasm.
Place, publisher, year, edition, pages
2000. Vol. 26, no 04-feb, 165-170 p.
penicillin amidase, proteolysis, E. coli, membrane transport, acylase, protein, degradation, proteases, invivo, expression, atcc-11105, stability, cells
IdentifiersURN: urn:nbn:se:kth:diva-19574ISI: 000085509700010OAI: oai:DiVA.org:kth-19574DiVA: diva2:338266
QC 201005252010-08-102010-08-10Bibliographically approved