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The relative importance of intracellular proteolysis and transport on the yield of the periplasmic enzyme penicillin amidase in Escherichia coli
KTH, Superseded Departments, Biotechnology.
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2000 (English)In: Enzyme and microbial technology, ISSN 0141-0229, E-ISSN 1879-0909, Vol. 26, no 04-feb, 165-170 p.Article in journal (Refereed) Published
Abstract [en]

Intracellular proteolysis is an important mechanism for regulating the level of the periplasmic enzyme penicillin amidase in Escherichia coli. Evidence is presented that the active enzyme is localized in the periplasmic space and maturation of pro-enzyme occurs during transport through the cytoplasmic membrane or rapidly after its entrance in the periplasm. The rate constants of the transport through cytoplasmic membrane and of the intracellular proteolysis were estimated to be 0.01 h and 0.5 h, respectively. This indicates that more than 90% of the synthesized pre-pro-enzyme is lost by intracellular proteolysis occurring in the cytoplasm.

Place, publisher, year, edition, pages
2000. Vol. 26, no 04-feb, 165-170 p.
Keyword [en]
penicillin amidase, proteolysis, E. coli, membrane transport, acylase, protein, degradation, proteases, invivo, expression, atcc-11105, stability, cells
URN: urn:nbn:se:kth:diva-19574ISI: 000085509700010OAI: diva2:338266
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

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