Gene regulation during late embryogenesis: the RY motif of maturation-specific gene promoters is a direct target of the FUS3 gene product
2000 (English)In: The Plant Journal, ISSN 0960-7412, E-ISSN 1365-313X, Vol. 21, no 5, 401-408 p.Article in journal (Refereed) Published
The Arabidopsis mutants fus3 and abi3 show pleiotropic effects during embryogenesis including reduced levels of transcripts encoding embryo-specific seed proteins. To investigate the interaction between the BO-domain-containing transcription factors FUSS and ABI3 with the RY cis-motif, conserved in many seed-specific promoters, a promoter analysis as well as band-shift experiments were performed. The analysis of promoter mutants revealed the structural requirements for the function of the RY cis-element. It is shown that both the nucleotide sequence and the alternation of purin and pyrimidin nucleotides (RY character) are essential for the activity of the motif. Further, it was shown that FUSS and ABI3 can act independently of each other in controlling promoter activity and that the RY cis-motif is a target for both transcription factors. For FUSS, which is so far the smallest known member of the B3-domain family, a physical interaction with the RY motif was established. The functional and biochemical data demonstrate that the regulators FUSS and ABI3 are essential components of a regulatory network acting in concert through the RY-promoter element to control gene expression during late embryogenesis and seed development.
Place, publisher, year, edition, pages
2000. Vol. 21, no 5, 401-408 p.
seed protein gene, conserved b3 domain, arabidopsis-thaliana, transgenic tobacco, leafy cotyledon1, storage protein, legumin gene, vicia-faba, embryo development, brassica-napus
IdentifiersURN: urn:nbn:se:kth:diva-19661ISI: 000086269900001OAI: oai:DiVA.org:kth-19661DiVA: diva2:338353
QC 201005252010-08-102010-08-10Bibliographically approved