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Recombinant expression of a selective blocker of M-1 muscarinic receptors
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2000 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 271, no 2, 435-439 p.Article in journal (Refereed) Published
Abstract [en]

Mamba venoms contain peptides with high selectivity for muscarinic receptors, Due to the limited availability of the M-1 muscarinic receptor-selective MT7 or ml-toxin 1, the peptide was expressed in Sf9 cells using a synthetic cDNA and purified, The isolated peptide had over four orders of magnitude higher affinity for the M-1 compared to M-2-M-5 muscarinic receptors, The peptide strongly inhibited Ca2+ mobilisation ;through recombinant and endogenously expressed M-1 receptors, having no effect on the function of the other subtypes. The MT7 peptide provides a unique tool for identification and functional characterisation of M-1 receptors in cells and tissues,

Place, publisher, year, edition, pages
2000. Vol. 271, no 2, 435-439 p.
Keyword [en]
muscarinic receptor, toxin, baculovirus, Sf9 cells, protein expression, amino-acid-sequence, mamba dendroaspis-angusticeps, acetylcholine-receptors, toxin, purification, m1-toxin, binding, venom
URN: urn:nbn:se:kth:diva-19748ISI: 000087000800029OAI: diva2:338440
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

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Jolkkonen, Mikael
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