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Mass transport limitations reduce the effective stereospecificity in enzyme-catalyzed kinetic resolution
KTH, Superseded Departments, Biochemistry and Biotechnology.
2000 (English)In: Organic Letters, ISSN 1523-7060, E-ISSN 1523-7052, Vol. 2, no 10, 1373-1376 p.Article in journal (Refereed) Published
Abstract [en]

The kinetic resolution of seudenol catalyzed by Candida antarctica lipase a in hexane was investigated. Large differences in reaction rate and stereospecificity were observed when different enzyme preparations were used, These differences were ascribed to mass transport limitations which reduced both reaction rate and stereospecificity. Lyophilized enzyme preparations were more apt to give this problem than immobilized preparations. Further, low substrate concentrations enhanced the effect. Thus, high alcohol concentrations and enzyme immobilization can be recommended.

Place, publisher, year, edition, pages
2000. Vol. 2, no 10, 1373-1376 p.
Keyword [en]
antarctica lipase-b, organic-solvents, enantiomeric ratio, nonaqueous media, biocatalysts, stability, reactors, seudenol, water
URN: urn:nbn:se:kth:diva-19752ISI: 000087018200008OAI: diva2:338444
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

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