Partial protection to respiratory syncytial virus (RSV) elicited in mice by intranasal immunization using live staphylococci with surface-displayed RSV-peptides
2000 (English)In: Vaccine, ISSN 0264-410X, E-ISSN 1873-2518, Vol. 18, no 24, 2743-2752 p.Article in journal (Refereed) Published
A live bacterial vaccine-delivery system based on the food-grade bacterium Staphylococcus carnosus was used for delivery of peptides from the G glycoprotein of human respiratory syncytial virus, subtype A (RSV-A). Three peptides, corresponding to the G protein amino acids, 144-159 (denoted G5), 190-203 (G9) and 171-188 (G4 S), the latter with four cysteine residues substituted for serines, were expressed by recombinant means as surface-exposed on three different bacteria, and their surface accessibility on the bacteria was verified by fluorescence-activated cell sorting (FACS). Intranasal immunization of mice with the live recombinant staphylococci elicited significant anti-peptide as well as anti-virus serum IgG responses of balanced IgG1/IgG2a isotype profiles, and upon viral challenge with 10(5) tissue culture infectious doses(50) (TCID50), lung protection was demonstrated for approximately half of the mice in the G9 and G4 S immunization groups. To our knowledge, this is the first study in which protective immunity to a viral pathogen has been evoked using food-grade bacteria as vaccine-delivery vehicles.
Place, publisher, year, edition, pages
2000. Vol. 18, no 24, 2743-2752 p.
live bacterial vaccine delivery, respiratory syncytial virus, Staphylococcus carnosus, recombinant lactococcus-lactis, streptococcal protein-g, albumin-binding region, g fusion protein, g-glycoprotein, cell-wall, klebsiella-pneumoniae, antibody-responses, oral vaccination, balb/c mice
IdentifiersURN: urn:nbn:se:kth:diva-19795ISI: 000087338600015OAI: oai:DiVA.org:kth-19795DiVA: diva2:338487
QC 201005252010-08-102010-08-10Bibliographically approved