Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Characteristics of a DO-controlled fed-batch culture of Escherichia coli
KTH, Superseded Departments, Biotechnology.
2000 (English)In: Bioprocess engineering (Berlin. Print), ISSN 0178-515X, E-ISSN 1432-0797, Vol. 22, no 6, 509-515 p.Article in journal (Refereed) Published
Abstract [en]

The DO-controlled glucose limited fed-batch technique was investigated in an E. coli process for production of a recombinant protein. The k(L)ac* value (oxygen transfer rate at zero oxygen concentration) was calculated from on-line gas analysis data during the process. In the investigated processes with induced production of recombinant protein, the k(L)ac* value decreased drastically several hours after induction. The reason for the decrease was found in increasing concentrations of DNA in the medium and increased viscosity due to cell lysis. The consequences of such a dramatic decrease in the volumetric oxygen transfer coefficient on the glucose feed and specific rates are described in computer simulations and experimental data.

Place, publisher, year, edition, pages
2000. Vol. 22, no 6, 509-515 p.
Keyword [en]
cultivation
Identifiers
URN: urn:nbn:se:kth:diva-19896ISI: 000088149100006OAI: oai:DiVA.org:kth-19896DiVA: diva2:338588
Note
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Enfors, Sven-Olof
By organisation
Biotechnology
In the same journal
Bioprocess engineering (Berlin. Print)

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 24 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf