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Characteristics of a DO-controlled fed-batch culture of Escherichia coli
KTH, Superseded Departments, Biotechnology.
2000 (English)In: Bioprocess engineering (Berlin. Print), ISSN 0178-515X, E-ISSN 1432-0797, Vol. 22, no 6, 509-515 p.Article in journal (Refereed) Published
Abstract [en]

The DO-controlled glucose limited fed-batch technique was investigated in an E. coli process for production of a recombinant protein. The k(L)ac* value (oxygen transfer rate at zero oxygen concentration) was calculated from on-line gas analysis data during the process. In the investigated processes with induced production of recombinant protein, the k(L)ac* value decreased drastically several hours after induction. The reason for the decrease was found in increasing concentrations of DNA in the medium and increased viscosity due to cell lysis. The consequences of such a dramatic decrease in the volumetric oxygen transfer coefficient on the glucose feed and specific rates are described in computer simulations and experimental data.

Place, publisher, year, edition, pages
2000. Vol. 22, no 6, 509-515 p.
Keyword [en]
URN: urn:nbn:se:kth:diva-19896ISI: 000088149100006OAI: diva2:338588
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

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