Change search
ReferencesLink to record
Permanent link

Direct link
Cooperative oligonucleotides in purification of cycle sequencing products
KTH, Superseded Departments, Biotechnology.ORCID iD: 0000-0001-8993-048X
Show others and affiliations
2000 (English)In: BioTechniques, ISSN 0736-6205, Vol. 29, no 2, 352-+ p.Article in journal (Refereed) Published
Abstract [en]

Nucleic acid hybridization is an essential component in many of today's standard molecular biology techniques. In a recent study, we investigated whether nucleic acid capture could be improved by taking advantage of stacking hybridization, which refers to the stabilizing effect that exists between oligonucleotides when they hybridize in a contiguous tandem fashion. Here, we describe a specific approach for purification of sequencing products using cooperative probes that hybridize to single-strand targets where one of the probes has been coupled to a magnetic bead. This approach has been developed for standard sequencing primers and has been applied to shotgun plasmid libraries. The cooperative probes have been designed to anneal within the common vector sequence and to avoid copurification of non extended sequencing primers and misprimed sequencing products. The reuse of magnetic beads, together with salt independent elution, makes the approach suitable for high-capacity capillary electrophoresis instruments.

Place, publisher, year, edition, pages
2000. Vol. 29, no 2, 352-+ p.
Keyword [en]
gene-expression patterns, c virus-rna, direct capture, dna, microarray
URN: urn:nbn:se:kth:diva-19956ISI: 000088679400031OAI: diva2:338648
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Uhlén, MathiasLundeberg, Joakim
By organisation
In the same journal

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 18 hits
ReferencesLink to record
Permanent link

Direct link