Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Solution properties of antitumor sulfated derivative of alpha-(1 -> 3)-D-glucan from Ganoderma lucidum
KTH, Superseded Departments, Biotechnology.ORCID iD: 0000-0001-9832-027X
Show others and affiliations
2000 (English)In: Bioscience, biotechnology and biochemistry, ISSN 0916-8451, E-ISSN 1347-6947, Vol. 64, no 10, 2172-2178 p.Article in journal (Refereed) Published
Abstract [en]

Four fractions of a water-insoluble alpha-(1-->3)-D-glucan GL extracted from fruiting bodies of Ganoderma lucidum were dissolved in 0.25 M LiCl/DMsO, and then reacted with sulfur trioxide-pyridine complex at 80 degreesC to synthesize a series of water-soluble sulfated derivatives S-GL. The degree of substitution of DS was measured by using IR infrared spectra, elemental analysis, and C-13 NMR to be 1.2-1.6 in the non-selective sulfation. Weight-average molecular weight M, and intrinsic viscosity [eta] of the sulfated derivatives S-GL were measured by multi-angle laser light scattering and viscometry. The M-w value (2.4 x 10(4)) of sulfated glucan S-GL-1 was much lower than that (44.5 x 10(4)) of original alpha-(1-->3)-D-glucan GL-1. The Mark-Houwink equation and average value of characteristic ratio C-infinity for the S-GL in 0.2 M NaCl aqueous solution at 25 degreesC were found to be: [eta] =1.32 x 10(-3) M-w(1.06) (cm(3) g(-1)) and 16, respectively, in the M-w range from 1.1 x 10(4) to 2.4 x 10(4). It indicated that the sulfated derivatives of the alpha-(1-->3)-D-glucan in the aqueous solution behave as an expanded chain, owing to intramolecular hydrogen bonding or interaction between charge groups. Interestingly, two sulfated derivatives synthesized from the alpha-(1-->3)-D-glucan and curdlan, a beta-(1-->3)-D-glucan, all had significant higher antitumor activity against Ehrlich ascites carcinoma (EAC) than the originals. The effect of expanded chains of the sulfated glucan in the aqueous solution on the improvement of the antitumor activity could not be negligible.

Place, publisher, year, edition, pages
2000. Vol. 64, no 10, 2172-2178 p.
Keyword [en]
Ganoderma lucidum, polysaccharide, sulfated modification, molecular weight, antitumor activity, anti-hiv activity, biological-activities, structural-analysis, molecular-weight, polysaccharides, conformation, (1->3)-beta-d-glucan, dependence, heparin, glucan
Identifiers
URN: urn:nbn:se:kth:diva-20141ISI: 000165100500021OAI: oai:DiVA.org:kth-20141DiVA: diva2:338834
Note
QC 20100525Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2017-12-12Bibliographically approved

Open Access in DiVA

No full text

Authority records BETA

Zhou, Qi

Search in DiVA

By author/editor
Zhou, Qi
By organisation
Biotechnology
In the same journal
Bioscience, biotechnology and biochemistry

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 25 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf