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Solution properties of antitumor sulfated derivative of alpha-(1 -> 3)-D-glucan from Ganoderma lucidum
KTH, Superseded Departments, Biotechnology.ORCID iD: 0000-0001-9832-027X
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2000 (English)In: Bioscience, biotechnology and biochemistry, ISSN 0916-8451, Vol. 64, no 10, 2172-2178 p.Article in journal (Refereed) Published
Abstract [en]

Four fractions of a water-insoluble alpha-(1-->3)-D-glucan GL extracted from fruiting bodies of Ganoderma lucidum were dissolved in 0.25 M LiCl/DMsO, and then reacted with sulfur trioxide-pyridine complex at 80 degreesC to synthesize a series of water-soluble sulfated derivatives S-GL. The degree of substitution of DS was measured by using IR infrared spectra, elemental analysis, and C-13 NMR to be 1.2-1.6 in the non-selective sulfation. Weight-average molecular weight M, and intrinsic viscosity [eta] of the sulfated derivatives S-GL were measured by multi-angle laser light scattering and viscometry. The M-w value (2.4 x 10(4)) of sulfated glucan S-GL-1 was much lower than that (44.5 x 10(4)) of original alpha-(1-->3)-D-glucan GL-1. The Mark-Houwink equation and average value of characteristic ratio C-infinity for the S-GL in 0.2 M NaCl aqueous solution at 25 degreesC were found to be: [eta] =1.32 x 10(-3) M-w(1.06) (cm(3) g(-1)) and 16, respectively, in the M-w range from 1.1 x 10(4) to 2.4 x 10(4). It indicated that the sulfated derivatives of the alpha-(1-->3)-D-glucan in the aqueous solution behave as an expanded chain, owing to intramolecular hydrogen bonding or interaction between charge groups. Interestingly, two sulfated derivatives synthesized from the alpha-(1-->3)-D-glucan and curdlan, a beta-(1-->3)-D-glucan, all had significant higher antitumor activity against Ehrlich ascites carcinoma (EAC) than the originals. The effect of expanded chains of the sulfated glucan in the aqueous solution on the improvement of the antitumor activity could not be negligible.

Place, publisher, year, edition, pages
2000. Vol. 64, no 10, 2172-2178 p.
Keyword [en]
Ganoderma lucidum, polysaccharide, sulfated modification, molecular weight, antitumor activity, anti-hiv activity, biological-activities, structural-analysis, molecular-weight, polysaccharides, conformation, (1->3)-beta-d-glucan, dependence, heparin, glucan
URN: urn:nbn:se:kth:diva-20141ISI: 000165100500021OAI: diva2:338834
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

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