Change search
ReferencesLink to record
Permanent link

Direct link
Selective receptor neurone responses to E-beta-ocimene, beta-myrcene, E,E-alpha-farnesene and homo-farnesene in the moth Heliothis virescens, identified by gas chromatography linked to electrophysiology
KTH, Superseded Departments, Chemistry.
2000 (English)In: Journal of Comparative Physiology A. Sensory, neural, and behavioral physiology, ISSN 0340-7594, E-ISSN 1432-1351, Vol. 186, no 9, 833-847 p.Article in journal (Refereed) Published
Abstract [en]

An important question in olfaction is for which odorants receptor neurones have evolved. In the present study, olfactory receptor neurones on the antennae of the tobacco budworm moth Heliothis virescens were screened for sensitivity to naturally occurring plant-produced volatiles by the use of gas chromatography linked to electrophysiology. Volatiles of host as well as non-host: plants collected by headspace techniques were used for stimulating the neurones, sequentially via two columns, one polar and one nonpolar installed in parallel in the gas chromatograph. Three types of neurones presented in this paper responded to one, two or three compounds for which the retention times were determined ill both column types. The chemical structures of the active components were determined on the basis of mass spectrometry linked to gas chromatography, indicating E-beta -ocimene and beta -myrcene as stimulants for neurone type 1, E,E,alpha -farnesene for neurone type 2 and homo-farnesene for neurone type 3. Re-testing authentic materials verified the identifications for the type I neurones. The results demonstrate a high specificity for the three types of neurones by strong responses to one or two structurally similar compounds out of hundreds present in a large variety of plants. The study exemplifies plant odour detection by narrowly tuned receptor neurones in a polyphagous moth species.

Place, publisher, year, edition, pages
2000. Vol. 186, no 9, 833-847 p.
Keyword [en]
insect-plant interactions, olfactory receptor neurones, plant odours, gas chromatography, single-cell recordings, induced volatile emissions, tobacco budworm moth, hylobius-abietis, plant volatiles, cotton plants, pine weevil, attractants, coleoptera, components, seedlings
URN: urn:nbn:se:kth:diva-20145ISI: 000165131000005OAI: diva2:338838
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Borg-Karlson, Anna-Karin
By organisation
In the same journal
Journal of Comparative Physiology A. Sensory, neural, and behavioral physiology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 15 hits
ReferencesLink to record
Permanent link

Direct link