Directed immobilization of recombinant staphylococci on cotton fibers by functional display of a fungal cellulose-binding domain
2001 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 195, no 2, 197-204 p.Article in journal (Refereed) Published
The immobilization of recombinant staphylococci onto cellulose fibers through surface display of a fungal cellulose-binding domain (CBD) was investigated. Chimeric proteins containing the CBD from Trichoderma reesei cellulase Cel6A were found to be correctly targeted to the cell wall of Staphylococcus carnosus cells. since full-length proteins could be extracted and affinity-purified. Furthermore. surface accessibility of the CBD was verified using a monoclonal antibody and functionality in terms of cellulose-binding was demonstrated in two different assays in which recombinant staphylococci were found to efficiently bind to cotton fibers. The implications of this strategy of directed immobilization Tor the generation of whole-cell microbial tools Fur different applications will be discussed.
Place, publisher, year, edition, pages
2001. Vol. 195, no 2, 197-204 p.
surface display, cellulose-binding domain, directed immobilization, Staphylococcus carnosus, reesei cellobiohydrolase-i, surface display, trichoderma-reesei, crystalline cellulose, cell-surface, protein-a, carnosus, peptide, subunit, hyicus
IdentifiersURN: urn:nbn:se:kth:diva-20384ISI: 000167087600015OAI: oai:DiVA.org:kth-20384DiVA: diva2:339079
QC 201005252010-08-102010-08-10Bibliographically approved