Determination of the maximum specific uptake capacities for glucose and oxygen in glucose-limited fed-batch cultivations of Escherichia coli
2001 (English)In: Biotechnology and Bioengineering, ISSN 0006-3592, E-ISSN 1097-0290, Vol. 73, no 5, 347-357 p.Article in journal (Refereed) Published
A simple pulse-based method for the determination of the maximum uptake capacities for glucose a nd oxygen in glucose limited cultivations of E. coli is presented. The method does not depend on the time-consuming analysis of glucose or acetate, and therefore can be used to control the feed rate in glucose limited cultivations, such as fed-batch processes. The application of this method in fed-batch processes of E. coli showed that the uptake capacity for neither glucose nor oxygen is a constant parameter, as often is assumed in fed-batch models. The glucose uptake capacity decreased significantly when the specific growth rate decreased below 0.15 h(-1) and fell to about 0.6 mmol g(-1) h(-1) (mmol per g cell dry weight and hour) at the end of fed-batch fermentations, where mu was approximately 0.02 h(-1). The oxygen uptake capacity started to decrease somewhat earlier when mu declined below 0.25 h(-1) and was 5 mmol g(-1) h(-1) at the end of the fermentations. The behavior of both uptake systems is integrated in a dynamic model which allows a better fitting of experimental values for glucose in fed-batch processes in comparison to generally used unstructured kinetic models.
Place, publisher, year, edition, pages
2001. Vol. 73, no 5, 347-357 p.
fed-batch-fermentation, kinetic model, acetate, oxygen uptake, dissolved oxygen tension, high-cell-density, recombinant protein-production, phenylalanine production, human interleukin-2, fermenter cultures, acetate formation, feeding strategy, growth, expression, metabolism
IdentifiersURN: urn:nbn:se:kth:diva-20612ISI: 000168612500003OAI: oai:DiVA.org:kth-20612DiVA: diva2:339308
QC 201005252010-08-102010-08-10Bibliographically approved