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Ultrasonic trapping in capillaries for trace-amount biomedical analysis
KTH, Superseded Departments, Physics.
KTH, Superseded Departments, Physics.ORCID iD: 0000-0003-2723-6622
2001 (English)In: Journal of Applied Physics, ISSN 0021-8979, E-ISSN 1089-7550, Vol. 90, no 1, 421-426 p.Article in journal (Refereed) Published
Abstract [en]

A longitudinal hemispherical standing-wave ultrasonic trap for size-selective separation of microspheres in small-diameter capillaries is described. The trap utilizes the competition between acoustic radiation forces and viscous drag forces on spheres suspended in a liquid inside 20-75-mum-diam fused silica capillaries. Experiments performed on 3.0- and 4.7-mum-diam latex spheres demonstrate the principles of trapping and verify the theoretically calculated size-dependent forces on the spheres. The spheres are detected by the use of laser-induced fluorescence. The goal is to use the trap for separation and ultrahigh-sensitivity detection of trace amounts of proteins and other macromolecules containing two antigenic sites, by binding the target molecule with high specificity to antibody-coated latex spheres.

Place, publisher, year, edition, pages
2001. Vol. 90, no 1, 421-426 p.
Keyword [en]
standing-wave, fluorescence detection, suspended particles, electrophoresis, compressibility, microparticles, droplet, fields
Identifiers
URN: urn:nbn:se:kth:diva-20717ISI: 000169361100064OAI: oai:DiVA.org:kth-20717DiVA: diva2:339413
Note
QC 20100525Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2017-12-12Bibliographically approved

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Hertz, Hans M.

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