Labeling of human C-peptide by conjugation with N-succinimidyl-4- F-18 fluorobenzoate
2001 (English)In: Journal of labelled compounds & radiopharmaceuticals, ISSN 0362-4803, E-ISSN 1099-1344, Vol. 44, no 7, 509-519 p.Article in journal (Refereed) Published
We have labeled proinsulin connecting peptide (C-peptide) with fluorine-18 (t(1/2) = 109.7min) in order to perform in vivo biodistribution and pharmacokinetic studies with position emission tomography (PET). This study reports the optimization of the conjugation labeling in the N-terminal with N-succinimidyl-4-[F-18]fluorobenzoate ([F-18]SFB). In preparative runs N-4-[F-18]fluorobenzoyl-C-peptide ([F-18]FB-C-peptide) was produced in 8-12% decay-corrected yields, counted from resolubilized [F-18]F-, in less than 5h. The specific radioactivity of [F-18]FB-C-peptide, determined using ELISA for one of the preparations, was around 70 GBq/mu mol at end of synthesis.
Place, publisher, year, edition, pages
2001. Vol. 44, no 7, 509-519 p.
fluorine-18, protein labeling, connecting peptide, diabetes, PET, n-succinimidyl 4-fluorobenzoate, f-18, proteins, exchange
IdentifiersURN: urn:nbn:se:kth:diva-20752ISI: 000169635200003OAI: oai:DiVA.org:kth-20752DiVA: diva2:339449
NoteQC 201005252010-08-102010-08-10Bibliographically approved