Efficient enantioselective separation of drug enantiomers by immobilised antibody fragments
2001 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 925, no 02-jan, 89-97 p.Article in journal (Refereed) Published
There is an increasing need for methods for efficient enantioselective separation and purification of chiral drugs. Genetic engineering provides the means for generating recombinant antibodies exhibiting extremely high specificity for even small molecular mass compounds. Here, recombinant antibody fragments have been generated for the drug diarylalkyltriazole that contains two chiral centres. Immobilised antibody fragments has been used successfully for efficient, step-wise separation of two enantiomers of the drug. Owing to the antibody specificity, one enantiomer came out in the flow-through, while the bound enantiomer could be specifically eluted. One of the antibodies tolerated solvents required both for dissolving the target molecules and for their elution for extended times and was shown to function over multiple cycles of the separation process.
Place, publisher, year, edition, pages
2001. Vol. 925, no 02-jan, 89-97 p.
enantiomer separation, immunoaffinity adsorbents, immobilised antibody fragments, cell-density fermentation, escherichia-coli, immunoaffinity chromatography, phage display, abscisic-acid, expression, miniantibodies, immobilization, mutagenesis, selection
IdentifiersURN: urn:nbn:se:kth:diva-20869ISI: 000170432100008OAI: oai:DiVA.org:kth-20869DiVA: diva2:339566
QC 201005252010-08-102010-08-10Bibliographically approved