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Catalytic mechanism of glyoxalase I: A theoretical study
2001 (English)In: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 123, no 42, 10280-10289 p.Article in journal (Refereed) Published
Abstract [en]

Hybrid density functional theory is used to study the catalytic mechanism of human glyoxalase I (GlxI). This zinc enzyme catalyzes the conversion of the hemithioacetal of toxic methylglyoxal and glutathione to nontoxic (S)-D-lactoylglutathione. GlxI can process both diastereomeric forms of the substrate, yielding the same form of the product. As a starting point for the calculations, we use a recent crystal structure of the enzyme in complex with a transition-state analogue, where it was found that the inhibitor is bound directly to the zinc by its hydroxycarbamoyl functions. It is shown that the Zn ligand Glu172 can abstract the substrate Cl proton from the S enantiomer of the substrate, without being displaced from the Zn ion. The calculated activation barrier is in excellent agreement with experimental rates. Analogously, the Zn ligand Glu99 can abstract the proton from the R form of the substrate. To account for the stereochemical findings, it is argued that the S and R reactions cannot be fully symmetric. A detailed mechanistic scheme is proposed.

Place, publisher, year, edition, pages
2001. Vol. 123, no 42, 10280-10289 p.
Keyword [en]
density-functional theory, protein environments, escherichia-coli, activation, reductase, accuracy, relevant, complex, model
Identifiers
URN: urn:nbn:se:kth:diva-21055ISI: 000171808300016OAI: oai:DiVA.org:kth-21055DiVA: diva2:339752
Note
QC 20100525Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2017-12-12Bibliographically approved

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