Change search
ReferencesLink to record
Permanent link

Direct link
Clue to damage recognition by UvrB: residues in the beta-hairpin structure prevent binding to non-damaged DNA
2001 (English)In: EMBO Journal, ISSN 0261-4189, E-ISSN 1460-2075, Vol. 20, no 21, 6140-6149 p.Article in journal (Refereed) Published
Abstract [en]

UvrB, the ultimate damage-recognizing component of bacterial nucleotide excision repair, contains a flexible beta -hairpin rich in hydrophobic residues. We describe the properties of UvrB mutants in which these residues have been mutated. The results show that Y101 and F108 in the tip of the hairpin are important for the strand-separating activity of UvrB, supporting the model that the beta -hairpin inserts between the two DNA strands during the search for DNA damage. Residues Y95 and Y96 at the base of the hairpin have a direct role in damage recognition and are positioned close to the damage in the UvrB-DNA complex. Strikingly, substituting Y92 and Y93 results in a protein that is lethal to the cell. The mutant protein forms pre- incision complexes on non-damaged DNA, indicating that Y92 and Y93 function in damage recognition by preventing UvrB binding to non-damaged sites. We propose a model for damage recognition by UvrB in which, stabilized by the four tyrosines at the base of the hairpin, the damaged nucleotide is flipped out of the DNA helix.

Place, publisher, year, edition, pages
2001. Vol. 20, no 21, 6140-6149 p.
Keyword [en]
damage recognition, DNA repair, nucleotide flipping, UvrB, nucleotide excision-repair, coli uvrabc endonuclease, escherichia-coli, crystal-structure, protein complex, thermus-thermophilus, preincision complex, (a)bc excinuclease, helicase, mechanism
National Category
Natural Sciences
URN: urn:nbn:se:kth:diva-21090ISI: 000172104000032OAI: diva2:339787
QC 20100525 NR 20140804Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2012-02-13Bibliographically approved

Open Access in DiVA

No full text

In the same journal
EMBO Journal
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 15 hits
ReferencesLink to record
Permanent link

Direct link