Screening and scanning of single nucleotide polymorphisms in the pig melanocortin 1 receptor gene (MC1R) by pyrosequencing
2001 (English)In: Animal Biotechnology, ISSN 1049-5398, E-ISSN 1532-2378, Vol. 12, no 2, 145-153 p.Article in journal (Refereed) Published
The increasing interest in the discovery and characterization of single nucleotide polymorphisms (SNPs) emphasis the need for high-throughput and cost effective scoring methods. Pyrosequencing is a novel method for screening SNPs. In this study we examine breed specific SNPs in the pig melanocortin I receptor gene (MC1R), some causing coat color phenotypes. A total of fifteen pigs representing eight breeds and crosses were analyzed by pyrosequencing. In addition to nine previously known SNPs, we also detected one new missense mutation by pyrosequencing. We here show that the SNPs were readily scored using standard reaction conditions. Insertions as well as substitutions were unambiguously detected and all genotypes were resolved in terms of homo- and heterozygozity.
Place, publisher, year, edition, pages
2001. Vol. 12, no 2, 145-153 p.
coat color, msh receptor, extension, pigmentation, mutations, locus
IdentifiersURN: urn:nbn:se:kth:diva-21236ISI: 000173122600006OAI: oai:DiVA.org:kth-21236DiVA: diva2:339934
QC 201005252010-08-102010-08-10Bibliographically approved