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Microarray data quality analysis: lessons from the AFGC project
KTH, Superseded Departments, Biotechnology.ORCID iD: 0000-0003-3281-8088
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2002 (English)In: Plant Molecular Biology, ISSN 0167-4412, E-ISSN 1573-5028, Vol. 48, no 02-jan, 119-131 p.Article in journal (Refereed) Published
Abstract [en]

Genome-wide expression profiling with DNA microarrays has and will provide a great deal of data to the plant scientific community. However, reliability concerns have required the development data quality tests for common systematic biases. Fortunately, most large-scale systematic biases are detectable and some are correctable by normalization. Technical replication experiments and statistical surveys indicate that these biases vary widely in severity and appearance. As a result, no single normalization or correction method currently available is able to address all the issues. However, careful sequence selection, array design, experimental design and experimental annotation can substantially improve the quality and biological of microarray data. In this review, we discuss these issues with reference to examples from the Arabidopsis Functional Genomics Consortium (AFGC) microarray project.

Place, publisher, year, edition, pages
2002. Vol. 48, no 02-jan, 119-131 p.
Keyword [en]
Arabidopsis, annotation, microarray functional genomics, normalization, differential gene-expression, dna-microarray, sequence tags, arabidopsis, discovery, patterns, hybridization, ontology, arrays
URN: urn:nbn:se:kth:diva-21250ISI: 000173211000008OAI: diva2:339948
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

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