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The use of flow cytometry to detect nucleic acids attached to the surface of Escherichia coli in high cell density fed-batch processes
KTH, Superseded Departments, Biotechnology.
2002 (English)In: Biotechnology letters, ISSN 0141-5492, E-ISSN 1573-6776, Vol. 24, no 3, 219-224 p.Article in journal (Refereed) Published
Abstract [en]

E. coli was grown in an aerobic fed-batch process for the production of a recombinant protein (rhGH). The cells were examined by flow cytometry and PI (propidium iodide) staining. The fluorescence of the PI-stained cells increased with increasing concentrations of DNA in the medium. Furthermore, DNA and RNA attached to the cell could partly be degraded with DNase/RNase and the fluorescence decreased. Formate excretion during the aerobic processes may be due to DNA and possibly also RNA attached to the cell surface, so creating diffusion resistance.

Place, publisher, year, edition, pages
2002. Vol. 24, no 3, 219-224 p.
Keyword [en]
DNA release, E. coli, flow cytometry, formate formation, culture, growth, w3110, flux
Identifiers
URN: urn:nbn:se:kth:diva-21312ISI: 000173802700011OAI: oai:DiVA.org:kth-21312DiVA: diva2:340010
Note
QC 20100525Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2017-12-12Bibliographically approved

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