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Changes in intracellular metabolite pools, and acetate formation in Escherichia coli are associated with a cell-density-dependent metabolic switch
KTH, Superseded Departments, Biotechnology.
KTH, Superseded Departments, Biotechnology.
2002 (English)In: Biotechnology letters, ISSN 0141-5492, E-ISSN 1573-6776, Vol. 24, no 6, 483-488 p.Article in journal (Refereed) Published
Abstract [en]

A cell density-dependent metabolic switch in amino acid metabolism occurs in E. coli W3110 batch cultures at 1.15 g dry wt l(-1) (Han L, Doverskog M, Enfors S-O, Haggstrom L, 2002, J. Biotechnol. 92: 237-249). A two- to three-fold decrease of the concentration of most glycolytic and citric acid cycle metabolites, and an increase in acetyl-CoA concentration after the switch, indicates that the central metabolism also is affected. The specific acetate production rate decreases throughout the culture, except for a temporary increase at the switch point. The intracellular acetate concentration remains relatively constant during the culture.

Place, publisher, year, edition, pages
2002. Vol. 24, no 6, 483-488 p.
Keyword [en]
acetate, Escherichia coli, intracellular metabolite pools, metabolic switch, quorum sensing, spectrophotometric assay, pyruvate-dehydrogenase, batch cultures, acetic-acid, growth, fermentation, coenzyme, overflow, lactate, glucose
URN: urn:nbn:se:kth:diva-21383ISI: 000174359600011OAI: diva2:340081
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

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