Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli
2002 (English)In: Biotechnology and applied biochemistry, ISSN 0885-4513, E-ISSN 1470-8744, Vol. 35, 91-105 p.Article, review/survey (Refereed) Published
Genetic strategies have been used for more than two decades to improve bacterial bioprocesses and to simplify recovery procedures. Such strategies include the design of efficient expression vectors and the improvement of bacterial production strains in different ways, e.g. by deletion of protease genes or engineering for overexpression of rare-codon tRNAs, foldases or chaperones. Gene multimerization is another such principle that has proved beneficial to improve production yields. Genetic strategies have furthermore been exploited to facilitate recovery processes by adapting the product for a particular purification principle. In this area, affinity fusions have been commonly used, but other principles, such as modified isoelectric point (pI) or hydrophobic properties have also been successfully investigated. A recent drastic step forward in the use of gene technology to improve recovery processes for recombinant proteins is the introduction of combinatorial protein engineering to generate tailor-made product-specific affinity ligands. This strategy, which allows efficient recovery of a recombinant protein in its native form, is likely to be increasingly used also in industrial-scale bioprocesses, since novel protein ligands have been described that can be sanitized using common industrial cleaning-in-p lace procedures. The examples presented in this review make it evident that genetic strategies will be of utmost importance in the future for facilitating production and recovery of recombinant proteins.
Place, publisher, year, edition, pages
2002. Vol. 35, 91-105 p.
affibody, affinity tag, fusion protein, in vitro refolding, soluble expression, high-level expression, high cell-density, aqueous 2-phase systems, maltose-binding protein, expanded-bed adsorption, growth-factor-i, bacterial alkaline-phosphatase, respiratory syncytial virus, solubilizing fusion partner, single-step purification
IdentifiersURN: urn:nbn:se:kth:diva-21460ISI: 000174962800004OAI: oai:DiVA.org:kth-21460DiVA: diva2:340158
QC 201005252010-08-102010-08-10Bibliographically approved