Bacterial growth and biofilm production on pyrene
2002 (English)In: FEMS Microbiology Ecology, ISSN 0168-6496, E-ISSN 1574-6941, Vol. 40, no 1, 21-27 p.Article in journal (Refereed) Published
Enrichment cultures inoculated with Arctic soil yielded a biofilm that grew on pyrene and phenanthrene. In a 60-day period, the biofilm degraded 20 mug ml(-1) pyrene or 39 mug ml(-1) phenanthrene. Single colonized pyrene crystals (approximately 1.5 x 0.75 X 0.35 mm) yielded 10(11) culturable heterotrophs and 10(5) biofilm propagules. Analysis of ribosomal intergenic spacers identified six phylotypes in a clone library from the pyrene biofilm. Analysis of 16S rRNA gene sequences indicated that the phylotypes. in order of decreasing abundance, are most closely related to members of the genera Polaromonas, Sphingomonas, Alcaligenes, Caulobacter and Variovorax. Two isolates capable of growth on pyrene. both Pseudomonas spp., were obtained from the pyrene enrichment culture. Growth of microbial biofilms on polycyclic aromatic hydrocarbons has not been reported previously, and this mode of growth may have important effects on substrate uptake.
Place, publisher, year, edition, pages
2002. Vol. 40, no 1, 21-27 p.
biodegradation, biofilm, polyaromatic hydrocarbon, pyrene, phenanthrene, polycyclic aromatic-hydrocarbons, polyaromatic hydrocarbons, degrading bacteria, biodegradation, degradation, bioavailability, mechanism, kinetics
IdentifiersURN: urn:nbn:se:kth:diva-21582ISI: 000175873400003OAI: oai:DiVA.org:kth-21582DiVA: diva2:340280
QC 201005252010-08-102010-08-10Bibliographically approved